Biowarfare: Experiment with Viruses that Destroy Bacteria

The goal of this science project is to experiment with the use of bacteriophage to kill E coli bacteria Bacteriophages (or phages for short) are small viruses that infect bacteria Because they can kill the bacteria that they infect it is possible that phages could be used to combat antibiotic-resistant strains of bacteria The phage you

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Bacteria Present In a Sample by Serial Dilution Agar

ADVERTISEMENTS: Total Plate Count (TPC): To enumerate bacteria present in a sample by serial dilution agar plating method or total plate count (TPC) method Purpose: The extent of bacterial activity in a given sample in a definite set of conditions mainly depends on the total number of bacteria present in it irrespective of their species

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INOCULATION OF AGAR PLATES USING A SWAB

INOCULATION OF AGAR PLATES USING A SWAB A sterile swab must be used care must be taken not to contaminate by touching other surfaces Transfer to the growth media as quickly as possible Depending on your purposes you can inoculate the swab in a few ways Described below is the Zigzag method of inoculation

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Growing bacteria in a petri dish {STEM activity for kids

Well I enlisted the help of my mother (a retired award winning science teacher) to help us culture and grow some bacteria Growing bacteria in a petri dish was a very impactful activity for both of my children making visible to them that which is usually invisible Note: Find more science experiments on my Science Activities for Kids page!

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MacConkey Agar

By using agar you can easily see the presence of contaminants and the kind of contaminants because they look distinct on the agar plate Agar is a polymer like gelatin but it has a better melting temperature when compared to the usual gelatin products The MacConkey agar can only develop gram negative bacteria

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Agar Cell Diffusion

Agar Cell Diffusion Use cubes of agar to investigate how cell size impacts diffusion All biological cells require the transport of materials across the plasma membrane into and out of the cell By infusing cubes of agar with a pH indicator and then soaking the treated cubes in vinegar you can model how diffusion occurs in cells

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How to Make Nutrient Agar at Home

How to Make Nutrient Agar at Home Inoculate the nutrient agar on the plate by wiping a swab containing the desired culture on the nutrient agar surface before covering with the lid Turn the petri dish over so that the solid is on top She has penned video scripts instructional manuals white papers and abstracts She has also

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Potato Dextrose Agar (PDA)

Aug 15 2019Potato Dextrose Agar (PDA) is used for the cultivation of fungi Potato Dextrose Agar (PDA) is a general purpose medium for yeasts and molds that can be supplemented with acid or antibiotics to inhibit bacterial growth It is recommended for plate count methods for foods dairy products and testing cosmetics

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Colony

Uses Colony-forming units are used to quantify results in many microbiological plating and counting methods including: The Pour Plate method wherein the sample is suspended in a Petri dish using molten agar cooled to approximately 40-45 C (just above the point of solidification to minimize heat-induced cell death)

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Bacteriophage Plaque Assay for Phage Titer (Theory

The main difference between Plaque Assay and Colony-Counting is that to count bacteria we spread about 100 - 400 bacteria over the surface of the agar and incubate the plate To count phage we spread 100 - 400 phage particles mixed with a very large number of host bacterial cells over the surface of the agar and incubate the plate

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Amazon: EZ BioResearch Bacteria Science Kit (I) : Pre

I was really happy with my purchase of petri dishes by EZ BioResearch Bacteria Science Kit I Used them for my thesis for my master program and they did a really great job at growing bacteria as you can see in the pictures provided They can with pipettes cotton swobs test tube sanitizers and 10 dishes with agar already in them

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Agar Cell Diffusion

Agar Cell Diffusion Use cubes of agar to investigate how cell size impacts diffusion All biological cells require the transport of materials across the plasma membrane into and out of the cell By infusing cubes of agar with a pH indicator and then soaking the treated cubes in vinegar you can model how diffusion occurs in cells

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Lab Report 1 Microbial Dispersion in the Environment

samples In this experiment agar plates were contaminated (i e colonies grew) when exposed to human breath only briefly With this in mind it is important for laboratory workers to use sterile technique when working with microorganisms in order to assure quality measurements In this experiment two different agars were used

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Amazon: Amazing Bacteria Science Kit

Easy to use prepoured agar plate bacteria growing set NO pouring your own plates which is time consuming and potentially dangerous and NO autoclaving This is an excellent science kit to learn microbiology and great for science fair projects Each plate is coated with a thick nutrient rich agar which bacteria molds and other fungi love!

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Optimization of single plate

We propose a simple technique for bacterial and yeast cfu estimations from diverse samples with no prior idea of viable counts designated as single plate-serial dilution spotting (SP-SDS) with the prime recommendation of sample anchoring (10 0 stocks) For pure cultures serial dilutions were prepared from 0 1 OD (10 0) stock and 20 μl aliquots of six dilutions (10 1 –10 6)

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LAB 4: ENUMERATION OF MICROORGANISMS

Slideshow: Dilutions: The Plate Count Method of Enumeration However if the sample is serially diluted and then plated out on an agar surface in such a manner that single isolated bacteria form visible isolated colonies the number of colonies can be used as a measure of the number of viable (living) cells in that known dilution However

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Amazon: EZ BioResearch Bacteria Science Kit (I) : Pre

I was really happy with my purchase of petri dishes by EZ BioResearch Bacteria Science Kit I Used them for my thesis for my master program and they did a really great job at growing bacteria as you can see in the pictures provided They can with pipettes cotton swobs test tube sanitizers and 10 dishes with agar already in them

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Interpreting Plates

What Can Grow on a Nutrient Agar Plate? Bacteria Each distinct circular colony should represent an individual bacterial cell or group that has divided repeatedly Being kept in one place the resulting cells have accumulated to form a visible patch Most bacterial colonies appear white cream or yellow in color and fairly circular in shape

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Potato Dextrose Agar (PDA)

Aug 15 2019Potato Dextrose Agar (PDA) is used for the cultivation of fungi Potato Dextrose Agar (PDA) is a general purpose medium for yeasts and molds that can be supplemented with acid or antibiotics to inhibit bacterial growth It is recommended for plate count methods for foods dairy products and testing cosmetics

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For Students – Science Education Enhances Knowledge (SEEK)

UV light is used as a control condition to show that the experiment works UV radiation causes mutations in the bacterial DNA and the colonies should turn white (UV light will also kill some of the colonies) What's not in the video: The preparation Prior to the actual experiment shown in the video the teacher carried out some preparation

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A Hand Washing Science Experiment

Agar plates were used for a hand-washing experiment Agar is used because it contains all the nutrients for the bacteria to grow such as salts yeast and amino acids The children thought the agar felt "gooey " The children first touched one side of their plate with unwashed hands

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Comparison of Liquid and Solid Bacterial Growth Media

Depending on the growth requirements of the bacteria and the information that the scientist hopes to gain various types of growth media are available for culturing bacteria Culturing means cultivating bacteria in a microbiology laboratory environment Article Summary: Culture media basically come in solid liquid form

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Techniques of isolation and Enumeration of Bacteria

For this reason the viable count is often called the plate count or colony count This method is used to enumerate bacteria in milk water foods soils cultures etc and the number of bacteria are expressed as colony-forming units (CFU) per ml

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Bacterial Growth Curve Analysis and its Environmental

Add 100 L of each dilution to the plate by pipetting the amount to the center of the agar plate (Figure 3) Immediately spread the aliquot by utilizing a flame sterilized "L" shaped glass rod On the day of the experiment pick a dilution for which there are between 30-300 colonies per plate Count the number of colonies on each

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