DMSO: Many Uses Much Controversy

DMSO: A History of Controversy The history of DMSO as a pharmaceutical began in 1961 when Dr Jacob was head of the organ transplant program at Oregon Health Sciences University It all started when he first picked up a bottle of the colorless liquid While investigating its potential as a preservative for organs he quickly discovered that

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MSM's secret cancer

MSM had cytotoxic effect on cancer cell lines but HepG2 cell line was more susceptible This study suggests that MSM may induce cytotoxic effect on gastrointestinal cancer cell lines by apoptosis and cell cycle arrest " 5 English: MSM killed stomach liver and esophageal cancer cells to varying degrees

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CATALOG NUMBER: AKR

this is not possible store at -80C until first culture Store subsequent cultured cells long term in liquid nitrogen QUANTITY CONCENTRATION: 1 mL 1 x 106 cells/mL in 70% DMEM 20% FBS 10% DMSO Background The human ovary cancer cell line SKOV-3 was derived from the ascitic fluid of a 64 year old Caucasian with an ovarian tumor in

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ECACC General Cell Collection: 98070106 HL60

References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line therefore further references should be obtained regarding cell culture characteristics Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated

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ECACC General Cell Collection: 88081201 THP 1

THP THP-1 THP1 Human monocytic leukaemia Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins These cells also stain positive for alpha-napthhyl butyrate esterase produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes)

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Dimethyl sulfoxide

Dimethyl sulfoxide (DMSO) is an organosulfur compound with the formula (CH 3) 2 S O This colorless liquid is an important polar aprotic solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water It has a relatively high boiling point

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CELL CULTURE BASICS

Cell Culture Cell culture is one of the major tools used in cellular and molecular biology providing excellent model systems for studying the normal physiology and biochemistry of cells (e g metabolic studies aging) the effects of drugs and toxic compounds on the cells

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Olaparib (AZD2281)

Olaparib is dissolved in DMSO and diluted by culture media before use The cells are seeded in six well plates and left to attach overnight Then Olaparib is added at various concentrations and the cells are incubated for 7-14 days After that the surviving colonies are

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Questionable Safety Of Dmso

08/04/2017When cancers were thawed with DMSO or dimethylformamide or butyrates they found what had been cancer in the deep-freeze it would revert to normal tissue in these structuring solvents Tumors were taken from two different colors of hamsters Cells isolated from the tumors mixed with a normal embryo – the developing embryo would normalize

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Store at

genomic instability and cell death (2 3) This mechanism of action has been shown to delay progression of the cell cycle through the late S and early G2 phase (4 5) Molecular Formula: C 29 H 32 O 13 Molecular Weight: 588 56 g/mol Solubility: Soluble in DMSO at 100 mg/ml very poorly soluble in ethanol and water with maximum solubility in

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dmso as a ligand cell

- if DMSO in required to solubilise the ligand add DMSO to the macromolecule solution to match immediately before running the experiment The recommended procedure is to make up the same volume of syringe and cell solution then add the same volume of DMSO to each using the same pipette Any small mismatch will give very large heats of dilution

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The Unbelievable Benefits of DMSO

DMSO has four main characteristics: What DMSO Does You can put a drop of DMSO on your big toe and taste it in your mouth in about 12 seconds It has a bit of a garlicky / oyster taste It goes through the cell walls of the body so fast that a dropper-full of it taken internally through the mouth never reaches the stomach when swallowed Most

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Domoso Solution for Animal Use

domoso solution is recommended for topical application only the application of domoso solution should take place only in well ventilated quarters inhalation of the drug should be avoided avoid contact of the medication with the eyes keep domoso solution out of the reach of children do not administer by any other route

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ECACC General Cell Collection: 88081201 THP 1

THP THP-1 THP1 Human monocytic leukaemia Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins These cells also stain positive for alpha-napthhyl butyrate esterase produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes)

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MSM's secret cancer

MSM had cytotoxic effect on cancer cell lines but HepG2 cell line was more susceptible This study suggests that MSM may induce cytotoxic effect on gastrointestinal cancer cell lines by apoptosis and cell cycle arrest " 5 English: MSM killed stomach liver and esophageal cancer cells to varying degrees

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Cell Culture

Cell Culture - Basics Techniques and Media - Essentially cell culture involves the distribution of cells in an artificial environment (in vitro) which is composed of the necessary nutrients ideal temperature gases pH and humidity to allow the cells to grow and proliferate In vivo - When the study involves living biological entities within the organism

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The Unbelievable Benefits of DMSO

DMSO has four main characteristics: What DMSO Does You can put a drop of DMSO on your big toe and taste it in your mouth in about 12 seconds It has a bit of a garlicky / oyster taste It goes through the cell walls of the body so fast that a dropper-full of it taken internally through the mouth never reaches the stomach when swallowed Most

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In Vitro Technologies :: Buffers and Supplements

Buffer cell culture media and solutions and support your bioprocessing needs using our buffers available at many scales Tailor your processes through our broad range of classical formulations or customized solutions Customize the culturing environment that best suits growth viability and cell health using process reagents and supplements

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Cell Culture Products Reviews Suppliers

Cell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined Accurately control your culture environment with bioreactors or culture incubators bind your cells to a surface or together with an extracellular matrix Distinguish cell

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An Ignored Natural Miracle Drug and It's Cancer

An Ignored Natural Miracle Drug and Its Cancer Treatment Applications by Paul Fassa See all TBYIL articles by Paul Fassa (The Best Years in Life) The Camelot Cancer Care in Tulsa Oklahoma was shut down in 2013 by the FDA and FBI They didn't use chemotherapy radiation or surgery and had a high cancer recovery rate

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DMSO: The Real Miracle Solution

DMSO also penetrates the skin and the blood-brain barrier with ease penetrating tissues and entering the bloodstream Furthermore DMSO protects the cells from mechanical damage and less of it is needed to achieve results as time passes as oppossed to most pharmaceuticals where increasing doses are required

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Cell culture preparation and plating protocol

Resuspend cell pellets in required volume of prewarmed culture media Plating out the cells Once the cells are resuspended in the correct amount of culture media make sure the cells are well mixed by using a cell scraper to scrape off any cells that may have settled in the flask while waiting for the count

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Knockout cell lines

How should I culture my cells? Please refer to the handling procedure on the KO cell line's datasheet and our cell culture protocol here What is the standard media formulation that your cells are in? The cells are in Cell Freezing Medium-DMSO Serum free media Please refer to datasheet for more information

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Guidelines for Use: Transwell Permeable Supports

Cells grown on permeable supports are often sensitive to initial seeding density for good cell attachment On first use try bracketing a range of seeding densities for optimum growth Cell attachment and spreading may be improved by pre-incubating permeable supports in medium prior to seeding

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DCTD Tumor Repository Catalog

T25 or T75 flask with 5-10% FBS and RPMI 1640 with L-glutamine or the recommended cell culture medium and incubated at the appropriate temperature and carbon dioxide level Each cell line must be passaged separately so as not to cross contaminate the lines Slowly increase the cell split ratios avoiding over-dilution which can impede cell

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